Notch信號靶標PCR芯片可用于研究Notch信號轉(zhuǎn)導的84個關(guān)鍵應(yīng)答基因的表達。Notch信號通路是一個傳統(tǒng)的發(fā)育途徑，參與細胞間通訊，細胞命運，細胞凋亡和發(fā)育。Notch信號通路首先在果蠅中發(fā)現(xiàn)，哺乳動物同源的Notch信號通路稍晚些被確定。來自Delta及Jagged/Serrate的配體可以激活與膜結(jié)合的Notch受體，誘導Notch胞內(nèi)結(jié)構(gòu)域斷裂。這種胞內(nèi)結(jié)構(gòu)域與RBPJ家族的轉(zhuǎn)錄因子以及共激活因子和輔阻遏物的相互作用來啟動靶基因的轉(zhuǎn)錄。Notch信號通路激活的輸出依賴于細胞內(nèi)容物，在適當?shù)臅r空調(diào)節(jié)激活是正常的胚胎發(fā)育過程所必須的。Notch信號通路失調(diào)可能會導致癌癥以及遺傳發(fā)育障礙。通過如ChIP和基因表達研究等實驗技術(shù)，很多經(jīng)典的Notch通路靶標基因都已經(jīng)被鑒定出來了。該芯片包含了Notch經(jīng)典信號通路的轉(zhuǎn)錄因子和研究驗證得到的高關(guān)聯(lián)性靶標基因，通過該芯片得到的結(jié)果可用于Notch信號傳導激活和抑制研究。通過實時定量PCR的方法，研究者即能夠利用該芯片簡單可靠地同時檢測Notch調(diào)節(jié)的細胞活動相關(guān)基因的表達。
Cell Fate Commitment: DKK1, HES1, HES5, JAG1, NODAL, NOTCH1, NOTCH3, PAX6, RUNX2, SOX9. Developmental Processes: Embryonic Development: DKK1, EDN1, EFNB1, FOXC1, FOXD3, FOXF1, HES1, HES7, MYF5, NODAL, NOTCH1, PBX1, PCDH8, PDGFRA, PDGFRB, RBPJ, TCF15, VEGFA, WNT4, WNT5A. Muscle Development: EDN1, EGR3, FOXC1, HBEGF (DTR), HEY2, IGFBP3, JAG1, MYF5, NOTCH1, PDGFB, PDGFRB, TNC. Epidermis Development: JAG1, KRT14, NOTCH1, PTGS2 (COX2), SNAI1 (SNAIL), SOX9, TCF15. Immune System Development: ID2, JAG1, JUN, KITLG, PBX1, RUNX1 (AML1), SGPL1, VEGFA. Nervous System Development: BDNF, CCND2, DKK1, DTX1, EFNA1, EFNB1, EGR3, FABP7, FOXC1, HES1, HES5, HEY1, HEY2, HEYL, ID3, ID4, JAG1, KALRN, NES, NODAL, NOTCH1, NOTCH3, PAX6, PBX1, RBPJ, RND1, VEGFA. Vasculature Development: EDN1, EFNA1, FLT1, FOXC1, FOXF1, HEY2, ID1, JAG1, JUN, NODAL, NOTCH1, RBPJ, RUNX1 (AML1), SGPL1, VEGFA. Kidney Development: ADAMTS1, BDNF, FOXC1, PBX1, PDGFRB, SGPL1. Other Development: NRARP, S1PR3. Signal Transduction: Notch Pathway: CREBBP (CBP), DTX1, FOXC1, HES1, HES7, HEY1, HEY2, HEYL, JAG1, LFNG, NOTCH1, NOTCH3, RBPJ, RBPJL, SNW1 (SKIIP). WNT Pathway: CCND1, DKK1, FRZB (FRP-3), FZD5, WISP1, WNT4, WNT5A, WNT6. Apoptosis: ABL1, BDNF, FOXC1, GADD45B, ID3, IGFBP3, JUN, KALRN, NOTCH1, PTCRA, PTGS2 (COX2), SGPL1, SOCS3, SOX9, VEGFA. Cytokines: CX3CL1, CXCL12 (SDF1), CXCL2, IL33, NAMPT. Transcription Factors: CBFA2T3CLOCK, CREBBP (CBP), EGR3, FOXC1, FOXD3, FOXF1, HEY1, HEY2, HEYL, JUN, MSC, MYF5, NOTCH1, PAX6, PBX1, RBPJ, RBPJL, RUNX1 (AML1), RUNX2, SOX9, TCF15. Other Genes: FJX1, GPSM2, HSPB8, MARK1, RHOV, TEC. |
