囊性纖維化PCR芯片可用于分析在囊性纖維化(CF)差異表達(dá)或與囊性纖維化跨膜電導(dǎo)調(diào)節(jié)(CFTR)相互作用的84個(gè)關(guān)鍵基因的表達(dá)。CFTR的突變會(huì)引起消化系統(tǒng)和呼吸系統(tǒng)的失調(diào),CF患者會(huì)有不同程度的支氣管粘液增厚,以及慢性肺炎導(dǎo)致的肺纖維化和肺功能衰竭目前的研究集中在患者逐漸惡化的CF肺部病變,通過(guò)治療可以延長(zhǎng)CF患者40年的平均壽命,但導(dǎo)致疾病及其癥狀的分子機(jī)制尚未發(fā)掘。這一芯片包含了離子轉(zhuǎn)運(yùn)蛋白基因、參與免疫或炎癥反應(yīng)的基因、可能與CFTR功能有關(guān)的基因。通過(guò)實(shí)時(shí)定量PCR的方法,研究者即能夠利用該芯片簡(jiǎn)單可靠地同時(shí)檢測(cè)CFTR分子機(jī)制相關(guān)基因的表達(dá)。 Modifier Genes: ADIPOR2, ADRB2, EDNRA, GCLC, GSTM1 (MGST1), IFRD1, IL8, MBL2, MSRA, NOS3 (eNOS), SCNN1B, SCNN1G, SERPINA1, TCF7L2, TGFB1, TNF, TNFRSF1A. Genes Interacting with CFTR: AHSA1, CANX, CFTR, DNAJA1, DNAJC5, EZR, GOPC, HSP90AA1, HSPA4 (HSP70), HSPA8, NME1 (NM23A), PDZK1, PPP2R4, PRKAA1, PRKAA2, SLC26A3, SLC9A3R1, SLC9A3R2, SNAP23, STX1A, STX8, TGFB1, TJP1, VCP. Neutrophil Chemotaxis: CCL2 (MCP1), CXCR2 (IL8RB), EDN1, EDNRA, IL1B, IL8, ITGB2. Inflammatory Response: ACE, ADRB2, ALOX12B, CCL2 (MCP1), CXCL1, CXCL2, DEFB1, EDNRA, IL10, IL1B, IL6, IL8, ITGB2, MBL2, NFKB1, PLA2G5, PTGS2 (COX2), S100A8, SERPINA1, TGFB1, TLR2, TLR4, TLR5, TNF, TNFRSF11A (RANK), TNFRSF1A. Immune Response: Innate: CCL2 (MCP1), CLU, DEFB1, ICAM1, LCN2 (NGAL), MAPK1 (ERK2), MBL2, NFKB1, NFKBIA (I?Ba), PRKCE, TGFB1, TLR2, TLR4, TLR5, TNFSF10 (TRAIL). Adaptive: CLU, FAS (TNFRSF6), ICAM1, IL10, IL1B, IL7R, MBL2, TGFB1, TLR4, TNF, TNFRSF11A (RANK). Unfolded Protein Response: CALR, DNAJA1, HSP90AA1, HSPA1A, HSPA4 (HSP70), HSPA8, HSPH1 (HSP105), VCP. Ion Binding & Transport: ADK, KCNE1, NR4A2 (NUR77), SCNN1B, SCNN1G, SLC9A3R1. Oxidative Stress: CLU, DUSP1 (PTPN16), GCLC, IL6, LCN2 (NGAL), MBL2, MSRA, NOS3 (eNOS), PTGS2 (COX2), SFTPB. Other Genes: EPSTI1, IGFBP5, ITGA2, KIT (CD117), MET, PRTN3, SLPI. |